By Roland Wagner, Hansjörg Hauser
This booklet introduces the basic rules and sensible software of concepts utilized in the scalable construction of proteins and virus-based gene-therapeutic items with animal telephone cultures. The ebook studies a vast spectrum of topics correct to biologics construction, together with the construction of telephone traces, a survey of sensible genomics for phone strains and methods, and advances in upstream and downstream approach improvement. it's a vital reference for all these operating within the biotechnology box either from an instructional and business point of view.
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Extra resources for Animal Cell Biotechnology
Proceedings of the National Academy of Sciences of the United States of America 1967;58:1205-12.  Ruley HE. Adenovirus early region 1A enables viral and cellular transforming genes to transform primary cells in culture. Nature 1983;304:602-6.  Barker DD, Berk AJ. Adenovirus proteins from both E1B reading frames are required for transformation of rodent cells by viral infection and DNA transfection. Virology 1987;156:107-21. 1 Generation of Cell Lines and Biotechnological Applications 31  Chinnadurai G.
As this characterization is dependent on the drug target and/or the desired indication there is no golden rule which cell systems are used in this stage of the drug development process. After narrowing down the number of potential drug candidates they are optimized with respect to efficacy and to pharmacokinetic properties like absorption, distribution, metabolism, excretion, and their toxicity profile (ADMET profiling). For some of these parameters robust cell based in vitro test systems have been established.
Nature Reviews Cancer 2010;10:550-60.  Bellacchio E, Paggi MG. Understanding the targeting of the RB family proteins by viral oncoproteins to defeat their oncogenic machinery. Journal of Cellular Physiology 2013;228:285-91.  Klingelhutz AJ, Foster SA, McDougall JK. Telomerase activation by the E6 gene product of human papillomavirus type 16. Nature 1996;380:79-82.  Halbert CL, Demers GW, Galloway DA. The E7 gene of human papillomavirus type 16 is sufficient for immortalization of human epithelial cells.
Animal Cell Biotechnology by Roland Wagner, Hansjörg Hauser