By Reinhard Neubert (Editor), Hans-Hermann Ruttinger (Editor)
This quantity offers breakthroughs and methods in affinity capillary electrophoresis to degree and verify the physicochemical and thermodynamic parameters of drug compounds. It discusses thoughts to discover and signify interactions to facilitate advancements in managed drug supply and focusing on.
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Extra resources for Affinity Capillary Electrophoresis in Pharmaceutics and Biopharmaceutics (Drugs and the Pharmaceutical Sciences: a Series of Textbooks and Monographs)
III. ALTERNATIVE ELECTROPHORETIC MODES A. Overview Capillary zone electrophoresis (CZE) is the most common electrophoretic separation technique due to its simplicity of operation and its ﬂexibility. It is the standard mode for drug analysis, identiﬁcation of impurities, and pharmacokinetic studies. Other separation modes, such as capillary isotachophoresis (CITP), micellar electrokinetc chromatography (MEKC), capillary electrochromatography (CEC), capillary gel electrophoresis (CGE), capillary isoelectric focusing, and afﬁnity capillary electrophoresis (ACE), have their advantages in solving speciﬁc separation problems, since the separation mechanism of each mode is different.
Nevertheless, the ability of a solvent to dissolve ionic species limits the number of solvents that can be used in CE. For instance, common buffer ions used in aqueous CE, such as phosphate and borate, cannot be employed Principles of Capillary Electrophoresis 21 in acetonitrile and alcohols. Therefore, in acetonitrile–methanol mixtures, acetic acid/ammonium acetate is widely used as background electrolyte. Also the selectivity of a separation can be increased by the addition of ions. Tjornelund and Hansen added magnesium ions to the buffer in order to separate tetracycline antibiotics in N-methyl formamide as metal chelates (12).
Fig. 8 Same conditions as in Fig. 5 mm. 42 IV. Ru¨ttinger CONCLUSIONS Afﬁnity capillary electrophoresis is a versatile method for the determination of binding constants. It needs only a very small amount of substance and, as a result of the high-resolution power of capillary zone electrophoresis, the method can even be applied for the determination of the afﬁnity of individual components of an crude mixture against a certain ligand. Hummel–Dreyer and vacancy analysis provide additional data from the peak area, whereas frontal analysis directly reads the concentrations from the detector response.
Affinity Capillary Electrophoresis in Pharmaceutics and Biopharmaceutics (Drugs and the Pharmaceutical Sciences: a Series of Textbooks and Monographs) by Reinhard Neubert (Editor), Hans-Hermann Ruttinger (Editor)